Researchers have found a new class of proteins in coral from Australia, which can block the HIV virus from penetrating T-cells; and, if used in gels or sexual lubricants, they may provide a powerful barrier against HIV infection.
There’s no question that coral reefs are a natural wonder. But now they may also be capable of stopping the spread of HIV. After screening thousands of natural product extracts in a biorepository maintained by the
The research team identified and purified the cnidarin proteins and then tested their activity against laboratory strains of HIV. The proteins proved very potent and capable of blocking HIV at concentrations of a billionth of a gram by preventing the first step in HIV transmission, in which the virus must enter a type of immune cell known as a T-cell.
Senior investigator Barry O’Keefe, Ph.D., deputy chief of the Molecular Targets Laboratory at the NCI’s Center for Cancer Research, said in a press release, “And the fact that this protein appears to block HIV infection—and to do it in a completely new way—makes this truly exciting. It’s always thrilling when you find a brand new protein that nobody else has ever seen before.”
In the global war against HIV, there has been interest in finding anti-HIV microbicides that women can apply to block HIV infection, without having to depend on a partner’s willingness to use a condom.
Koreen Ramessar, Ph.D., a postdoctoral research fellow at the NCI and a member of the research team, said cnidarins could be ideally suited for use in such a product because the proteins block HIV transmission without encouraging the virus to become resistant to other HIV drugs.
“When developing new drugs, we’re always concerned about the possibility of undermining existing successful treatments by encouraging drug resistance in the virus,” said O’Keefe, in the press statement.
O’Keefe continued, “But even if the virus became resistant to these proteins, it would likely still be sensitive to all of the therapeutic options that are currently available.”
“We found that cnidarins bind to the virus and prevent it from fusing with the T-cell membrane,” said Ramessar. “This is completely different from what we’ve seen with other proteins, so we think the cnidarin proteins have a unique mechanism of action.”
The researchers’ next step is to refine methods for generating cnidarins in larger quantities so the proteins can be tested further to identify potential side effects or activity against other viruses.
“Making more of it is a big key. You can’t strip the Earth of this coral trying to harvest this protein, so our focus now is on finding ways to produce more of it so we can proceed with preclinical testing,” said O’Keefe.
The scientists discovered cnidarins while screening for proteins, a largely understudied component of natural product extracts found in the National Cancer Institute’s extract repository. The institute maintains a large collection of natural specimens gathered from around the world under agreements with their countries of origin. The specimens are available to researchers across the United States.
“The natural products extract repository is a national treasure,” said O’Keefe. “You never know what you might find. Hopefully, discoveries like this will encourage more investigators to use this resource to identify extracts with activity against infectious disease.”
The study findings were featured at the Experimental Biology 2014 meeting of The American Physiological Society in San Diego on April 29, 2014.