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Even the leanest, healthiest athletes have a substantial energy pool of stored lipids. The average storage in adipose tissue ranges between 50,000 and 100,000 calories, or enough energy to walk or run 500 to 1,000 miles (800 to 1,600 kilometers) without a refueling stop. In addition, athletes store approximately 2,000 to 3,000 calories of lipids inside the muscle tissue. These lipids, which are stored in the form of triglycerides, are available as a fuel under the proper conditions of oxygen availability. Maximal fat oxidation occurs at 60 to 65 percent VO2 max, but at higher levels of VO2max there is insufficient oxygen to derive the majority of energy used from fat catabolism.
Triglycerides stored in adipose tissue are cleaved into their component molecules of glycerol and fatty acids and transported to the blood plasma. The glycerol is available to all tissues for energy metabolism, and the free fatty acids are transported to working muscles where they are oxidized for energy. The triglycerides stored in working muscles are cleaved into glycerol and fatty acids, and the fatty acids can be oxidized for energy where they are resident. The glycerol can also be burned for energy in the working muscle or can be transported to the blood plasma as a source of energy for other tissues.
The lower the exercise intensity, the greater the proportion of fat burned to satisfy energy needs. As exercise intensity increases, the proportion of fat burned decreases and the proportion of carbohydrate burned increases. It is this basic reality that is behind why so many people do low-intensity activity to burn fat and lower body fat levels. However, the proportion of fat burned should not be confused by the total amount of fat burned at different intensities of physical activity. As exercise intensity increases, the total number of calories burned per unit of time also increases. Although there may be a decrease in the proportion of fat burned to satisfy total energy needs in higher-intensity activity, the total volume of fat burned is greater because the total energy requirement is higher. The take-away message from this metabolic reality is that athletes interested in lowering body fat should exercise at least as high as 65 percent of VO2max for the duration of their workouts to optimize the total mass of fat that is burned. Exercising at lower intensities burns a greater proportion of fat but less total fat than exercising at higher intensities.